Multicolour immunohistochemistry for
4 biomarkers of breast cancer

Marker Detection Color
Her2 DAB Brown
ER Tyr-Tamra Magenta
PR QM-Cy5 Cyan
Ki67 QM-Dabsyl Yellow
Counterstain Hem II Light blue
Tissue Breast  
Primary antibodies hapten labeled
Three enzymes (HRP, AP, β-Gal)
Green = QM-Dabsyl + QM-Cy5